Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.
نویسندگان
چکیده
The thymidylate synthetase gene of B. subtilis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9 is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi-3-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3-T, but not those of plasmid vectors.
منابع مشابه
8. Cloning of the Thymidylate Synthetase Gene of the Phage Phi-3-T
Phi-3-T is a Bacillus subtifis temperate bacteriophage, isolated by Tucker (11) from the soil. Tucker has shown that infection by this phage causes, by lysogenic conversion of thyB. subtifis clones, prototrophy. The phage carries genetic information specifying the thymidylate synthetase activity, the thyP gene. Recently, we have reported purification of B. subtilis DNA segments achieved by EcoR...
متن کاملExpression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi - 3 - T in Escherichia coli ( DNA cloning / plasmid / transformation / hybridization )
The thymidylate synthetase gene of B. subtifis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9, is expressed in E. colt. The promoter of the cloned tene is likely to originate in Phi4-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtifis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3T, but not those...
متن کاملExpression of the Bacillus subtilis glutamine synthetase gene in Escherichia coli.
The structural gene for glutamine synthetase (glnA) in Bacillus subtilis ( glnAB ) cloned in the lambda vector phage Charon 4A was used to transduce a lysogenic glutamine auxotrophic Escherichia coli strain to prototrophy. The defective E. coli gene ( glnAE ) was still present in the transductant since it could be transduced. In addition, curing of the prototroph resulted in the restoration of ...
متن کاملMOLECULAR CLONING AND EVALUATION OF WILD PROMOTER IN EXPRESSION OF BACILLUS SPHAERICUS PHENYLALANINE DEHYDROGENASE GENE IN BACILLUS SUBTILIS CELLS
To evaluate the role of wild promoter of L-phenylalanine dehydrogenase (PheDH) gene, referred to as pdh, from Bacillus sphaericus in expression, cloning of pdh gene in Bacillus subtilis was performed. The whole pdh gene was cloned in pHY300PLK shuttle vector and amplified, construct (pHYDH) then transformed in B. subtilis ISW1214 and E. coli JM109. The pdh endogenous promoter presented no effec...
متن کاملCloning of Pf3, a filamentous bacteriophage of Pseudomonas aeruginosa, into the pBD214 vector of Bacillus subtilis.
The genome of Pf3, a filamentous single-stranded DNA bacteriophage of Pseudomonas aeruginosa (a gram-negative organism) was cloned into pBD214, a plasmid cloning vector of Bacillus subtilis (a gram-positive organism). Cloning in the gram-positive organism was done to avoid anticipated lethal effects. The entire Pf3 genome was inserted in each orientation at a unique Bc/I site within a thymidyla...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 73 11 شماره
صفحات -
تاریخ انتشار 1976